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il 15  (R&D Systems)


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    Structured Review

    R&D Systems il 15
    Il 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+il+15/Recombinant+Human+IL-15+Protein/bio_rxiv__64898__2026__04__21__719872-209-14-15
    Average 95 stars, based on 200 article reviews
    il 15 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18
    Article Snippet: Cells were analyzed by flow cytometry (BD Fortessa, BD Biosciences) and FlowJo software. .. Freshly isolated NK cells (2 × 10 6 /ml), NK-92 cells (5 × 10 5 /ml), or KHYG-1 cells (5 × 10 5 /ml) were cultured in 10 ng/ml human recombinant IL-15 alone, or in combination with 10 ng/ml human recombinant IL-12 and IL-18 (all from R&D Systems) in serum-free AIM-V medium for 48 h. Additionally, NK cells were stimulated with anti-CD16-coated pan-mouse Dynabeads (ThermoFisher Scientific) for 48 h in AIM-V medium. ..

    Article Title: Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity.
    Article Snippet: .. PBMC from one control were rested overnight with 10 ng/ml human recombinant IL-15 (R&D Sytems) and NK cells were then isolated using EasySep Human NK Cell Isolation Kit (Stemcell Technologies). .. Nunc MaxiSorp Flat-Bottom plates (ThermoFisher) were coated with 1.5 ng/ well of SARS-CoV-1 or SARS-CoV-2 spike proteins and incubated at 4 C for 6 h, then washed and blocked with R10 for 19 h at 4 C. After blocking, 100 ul of vNARs (5ug/ml) were added to each well and plates were incubated 30min at 37 C. Plates were then washed with PBS 4x, followed by addition of 25,000 purified NK cells per well.

    Article Title: Isolation of a cytolytic subpopulation of extracellular vesicles derived from NK cells containing NKG7 and cytolytic proteins.
    Article Snippet: .. NK-92 cells (1x106/ml) were cultured with 10 ng/ml human recombinant IL-15, while freshly isolated primary NK cells (2x106/ ml) were cultured in 10 ng/ml of IL-12, IL-15 and IL-18 (R&D Systems) in serum-free AIM-V medium for 48 hrs. ..

    Cell Culture:

    Article Title: Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18
    Article Snippet: Cells were analyzed by flow cytometry (BD Fortessa, BD Biosciences) and FlowJo software. .. Freshly isolated NK cells (2 × 10 6 /ml), NK-92 cells (5 × 10 5 /ml), or KHYG-1 cells (5 × 10 5 /ml) were cultured in 10 ng/ml human recombinant IL-15 alone, or in combination with 10 ng/ml human recombinant IL-12 and IL-18 (all from R&D Systems) in serum-free AIM-V medium for 48 h. Additionally, NK cells were stimulated with anti-CD16-coated pan-mouse Dynabeads (ThermoFisher Scientific) for 48 h in AIM-V medium. ..

    Article Title: Isolation of a cytolytic subpopulation of extracellular vesicles derived from NK cells containing NKG7 and cytolytic proteins.
    Article Snippet: .. NK-92 cells (1x106/ml) were cultured with 10 ng/ml human recombinant IL-15, while freshly isolated primary NK cells (2x106/ ml) were cultured in 10 ng/ml of IL-12, IL-15 and IL-18 (R&D Systems) in serum-free AIM-V medium for 48 hrs. ..

    Recombinant:

    Article Title: Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18
    Article Snippet: Cells were analyzed by flow cytometry (BD Fortessa, BD Biosciences) and FlowJo software. .. Freshly isolated NK cells (2 × 10 6 /ml), NK-92 cells (5 × 10 5 /ml), or KHYG-1 cells (5 × 10 5 /ml) were cultured in 10 ng/ml human recombinant IL-15 alone, or in combination with 10 ng/ml human recombinant IL-12 and IL-18 (all from R&D Systems) in serum-free AIM-V medium for 48 h. Additionally, NK cells were stimulated with anti-CD16-coated pan-mouse Dynabeads (ThermoFisher Scientific) for 48 h in AIM-V medium. ..

    Article Title: Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity.
    Article Snippet: .. PBMC from one control were rested overnight with 10 ng/ml human recombinant IL-15 (R&D Sytems) and NK cells were then isolated using EasySep Human NK Cell Isolation Kit (Stemcell Technologies). .. Nunc MaxiSorp Flat-Bottom plates (ThermoFisher) were coated with 1.5 ng/ well of SARS-CoV-1 or SARS-CoV-2 spike proteins and incubated at 4 C for 6 h, then washed and blocked with R10 for 19 h at 4 C. After blocking, 100 ul of vNARs (5ug/ml) were added to each well and plates were incubated 30min at 37 C. Plates were then washed with PBS 4x, followed by addition of 25,000 purified NK cells per well.

    Article Title: The IL-15 / sIL-15Rα complex modulates immunity without effect on asthma features in mouse
    Article Snippet: .. Human recombinant IL-15 (12.9 k Dalton, Peprotech France) and human IL-15 Rα-Fc (42.6 k Dalton R & D Systems) were reconstituted in PBS at concentrations of 10 μg/mL and 60 μg/mL respectively. ..

    Article Title: Circulating cancer-specific CD8 T cell frequency is associated with response to PD-1 blockade in Merkel cell carcinoma
    Article Snippet: All single-cell suspensions were cryopreserved in Freezing Medium [50% human serum (Valley Biomedical), 40% RPMI (Corning), and 10% DMSO (Sigma-Aldrich)] and stored in liquid nitrogen. .. To expand T cells from tumors, tumor tissue was chopped into small pieces and placed in culture along with 10 6 allogenic irradiated PBMCs, Remel phytohemagglutinin-purified mitogen (1.6 μg/mL; ThermoFisher), human recombinant IL-15 (10 ng/mL; R&D Systems), and human natural interleukin IL-2 (32 U/mL; Hemagen, Columbia, MD). ..

    Article Title: HIV-1-mediated downmodulation of HLA-C impacts target-cell recognition and antiviral activity of NK cells
    Article Snippet: .. NK cells were resuspended in complete medium supplemented with 1 ng/ml human recombinant IL-15 (hrIL-15; R&D systems). ..

    Article Title: Tolerogenic dendritic cells, method for their production and uses therof
    Article Snippet: .. All the experiments were conducted in the presence of recombinant human IL-2 (100 U/ml) (Chiron) and human recombinant IL-15 (1 ng/ml) (R&D) (obtained cells are named Th0 cells). .. In addition, the following soluble factors were added as indicated: rhIL-10 (10 ng/ml) (BD, Bioscience), rh-IFN-α (5 ng/ml) (R&D) (obtained cells are named Tr1 cells), soluble HLA-G1 (30 ng/ml) alone (obtained cells are named Tg) or in combination with rhIL-10 (10 ng/ml) (obtained cells are named Tg10).

    Article Title: Isolation of a cytolytic subpopulation of extracellular vesicles derived from NK cells containing NKG7 and cytolytic proteins.
    Article Snippet: .. NK-92 cells (1x106/ml) were cultured with 10 ng/ml human recombinant IL-15, while freshly isolated primary NK cells (2x106/ ml) were cultured in 10 ng/ml of IL-12, IL-15 and IL-18 (R&D Systems) in serum-free AIM-V medium for 48 hrs. ..

    Control:

    Article Title: Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity.
    Article Snippet: .. PBMC from one control were rested overnight with 10 ng/ml human recombinant IL-15 (R&D Sytems) and NK cells were then isolated using EasySep Human NK Cell Isolation Kit (Stemcell Technologies). .. Nunc MaxiSorp Flat-Bottom plates (ThermoFisher) were coated with 1.5 ng/ well of SARS-CoV-1 or SARS-CoV-2 spike proteins and incubated at 4 C for 6 h, then washed and blocked with R10 for 19 h at 4 C. After blocking, 100 ul of vNARs (5ug/ml) were added to each well and plates were incubated 30min at 37 C. Plates were then washed with PBS 4x, followed by addition of 25,000 purified NK cells per well.

    Cell Isolation:

    Article Title: Shark nanobodies with potent SARS-CoV-2 neutralizing activity and broad sarbecovirus reactivity.
    Article Snippet: .. PBMC from one control were rested overnight with 10 ng/ml human recombinant IL-15 (R&D Sytems) and NK cells were then isolated using EasySep Human NK Cell Isolation Kit (Stemcell Technologies). .. Nunc MaxiSorp Flat-Bottom plates (ThermoFisher) were coated with 1.5 ng/ well of SARS-CoV-1 or SARS-CoV-2 spike proteins and incubated at 4 C for 6 h, then washed and blocked with R10 for 19 h at 4 C. After blocking, 100 ul of vNARs (5ug/ml) were added to each well and plates were incubated 30min at 37 C. Plates were then washed with PBS 4x, followed by addition of 25,000 purified NK cells per well.

    Irradiation:

    Article Title: Circulating cancer-specific CD8 T cell frequency is associated with response to PD-1 blockade in Merkel cell carcinoma
    Article Snippet: All single-cell suspensions were cryopreserved in Freezing Medium [50% human serum (Valley Biomedical), 40% RPMI (Corning), and 10% DMSO (Sigma-Aldrich)] and stored in liquid nitrogen. .. To expand T cells from tumors, tumor tissue was chopped into small pieces and placed in culture along with 10 6 allogenic irradiated PBMCs, Remel phytohemagglutinin-purified mitogen (1.6 μg/mL; ThermoFisher), human recombinant IL-15 (10 ng/mL; R&D Systems), and human natural interleukin IL-2 (32 U/mL; Hemagen, Columbia, MD). ..



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